Manufacturer: SCIEX, USA

Mass spectrometer/ Analyser: A High end Sensitive LC/MS/MS system for new born screening, Therapeutic drug monitoring and other clinical tests, and other biological sample applications along with UHPLC and other accessories. The system should meet following minimum specifications.

Ionization Source: System should have ionization source capable of (ESI) & APCI).The source will have a flow rate compatibility in ESI mode from 5 micro L/min to 3000 μL/min and APCI mode from 50 microL/min to 3000 μL/min; without flow splitting. There should be a provision available to divert the flow to waste/MS through software before/during/after the analysis in order to reduce source fouling/contamination. The desolvation temperature will be user selectable from ambient to 750 deg Celsius.

The Mass Range: Minimum 5 -2000 amu or better Mass Stability 0.1 Da over 24 hours , MRM dwell time 1 msec Sensitivity ESI +ve using 1pg of reserpine on column S/N > 150,000 or better , ESI -ve using 1pg of chloramphenicol on column S/N > 150,000or better APCI +ve mode sensitivity for reserpine (10 pg/uL, 5 uL fixed loop injection), monitoring 609/195 MRM transition, the instrument must have a S/N > 40,000:1, Scan speed 20000 or better,

Dynamic range: 5 orders of magnitude or better

Operating modes: Mass spectrometer should have the following

scan options: Full scan, Product ion scan, Precursor ion scan, Neutral loss scan, Multiple Reaction Monitoring (MRM), MS3 and MRM3, Enhanced MS Scan, Enhanced Product Ion Scan, Enhanced Resolution Scan,

Nitrogen generator and air compressor: Nitrogen Generator with noise free compressor. System should work on Nitrogen/Zero air/zero grade air/dry air only without need of external Argon or helium gas for collision cell.

Computer system: Suitable Computer system with required software. The system must be compatible with a variety of commercially available LC pumps, autosamplers, manual injectors and detectors. Software is designed for data processing to quickly screen compounds in a large set data generated in Software by flow-injection triple quadrupole mass spectrometry (FI/MS/MS). The concentrations for all compounds are determined. If the acceptance limits for the compounds have been established, the program highlights the samples having results outside of the limits. UHPLC System Analytical UHPLC system (Equipped with temperature controlled Auto-sampler, thermostat column compartment, Degasser).

UHPLC Binary Pump: Flow Rate Range at least in - 0.01 to 10.0000 mL/min or better and suitable for LC-MS/MS operation. Should have Maximum Pressure- 18800 PSI or better. Autosampler with sample cooler Autosampler should be available with a capacity of minimum 105 vials or more of vials with capacity ~1.5 ml vial or better capacity. Accurate and precise injection volume from 1 µl to 50µl. Temperature controlled auto-sampler compartment (4-40°C). Injection Volume Precision- RSD ≤ 0.3% or better. Carryover- Less than 0.0015% (15 ppm). Rack for 96 well plates should be supplied.

Column Oven: Column Oven- Room Temperature to 150°C or better UPS: Online UPS of 10 Kva capacity with at least 60 mins back up for the complete system should be provided.