Principles of the Procedure

Soyabean Casein Digest Agar Plate contains pancreatic digest of casein and papaic digest of soyabean which provides amino acids, long chain peptides and essential nutrients required for the growth of microorganisms. Sodium chloride maintains the osmotic balance. Haemophilus species may be differentiated by their requirements for X and V factors. Paper strips impregnated with these factors are placed on the surface of the medium after inoculation with the test organism. Following incubation, a zone of growth around the strip indicates a requirement for the factor(s).

Formula / Liter

Precautions

1.    Prepared plated media are For in vitro Diagnostic Use or For Laboratory Use as labeled.

2.   Directions for use should be read and followed carefully.

3.   If excessive moisture is observed, invert the bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation.

Observe aseptic techniques and established precautions against microbiological hazards throughout all procedures, since it must be assumed that all specimens/samples collected might contain infectious microorganisms.

Product Deterioration

Do not use plates if they show evidence of microbial contamination, discoloration, drying, cracking or other signs of deterioration.

Directions

Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.

Quality Control Specifications

Dose of irradiation : 10.00- 25.00

Sterility Check: Passes release criteria.

Cultural Response

Recovery rate is considered 100% for bacteria growth on Blood Agar and fungus growth on Sabouraud Dextrose Agar.