Principles of the Procedure

Rappaport Vassiliadis Soya Broth (RVS Broth) contains soya peptone that contains natural sugars which provide essential growth nutrients and enhance the growth of Salmonella.⁽¹⁰⁾ Magnesium chloride raises the osmotic pressure in the medium. Phosphates act as buffering agents in the medium to maintain the constant pH. Sodium chloride maintains the osmotic balance. Malachite green is inhibitory to organisms other than Salmonellae. The low pH of the medium, combined with the presence of malachite green and magnesium chloride, helps to select for the highly resistant Salmonella species. The relatively lower concentration of nutrition, also aids selective enrichment of Salmonella.