Intended Use
Blood Culture Products are recommended for detection of microorganisms in blood samples.
Product Summary and Explanation
Blood is one of the most important specimens received by the laboratory and blood culture is one of the most important and critical procedures performed in the microbiology laboratory. The isolation and identification of an organism has great diagnostic significance, blood being normally sterile. Blood cultures are of great importance in diagnosing such conditions as endocarditis, typhoid fever, pneumonia, supportive thrombophlebitis, infection of vascular grafts and other disease characterized by bacteremia.(1)
Principles of the Procedure
Blood is collected from the patient (preferably before antibiotic therapy is initiated) by venipuncture with a needle and syringe (or blood collecting set) and immediately transferred aseptically to Micromaster Blood Culture bottle containing
the desired growth medium. The bottle is then incubated and can be observed for turbidity, color change, hemolysis, gas formation or other evidence of microbial growth. Appropriate conventional subculturing methods should be used.
Formula / Lit
Brain Heart Infusion Broth (BHI Broth)
|
Ingredients |
Gms/Liter |
|
Brain Heart Infusion Broth (DM811) |
37.00 |
|
SPS |
0.25 |
|
Final pH ( at 25°C) 7.4 ± 0.2 |
|
BHI Broth-Supplemented w/0.05%SPS
|
Ingredients |
Gms/Liter |
|
Brain Heart Infusion Broth (DM811) |
37.00 |
|
SPS |
0.50 |
|
Yeast Extract |
4.00 |
|
Pyridoxal HCl |
0.005 |
|
Menadione Sodium Bisulfite |
2.50 |
|
Final pH ( at 25°C) 7.4 ± 0.2 |
|
Tryptone Soya Broth (TSB)
|
Ingredients |
Gms/Liter |
|
Tryptone Soya Broth (DM277) |
30.00 |
|
SPS |
0.25 |
|
pH after sterilization ( at 25°C) 7.3 ± 0.2 |
|
Tryptone Soya Broth Supplemented w/0.05%SPS
|
Ingredients |
Gms/Liter |
|
Tryptone Soya Broth (DM277) |
30.00 |
|
Yeast Extract |
2.50 |
|
SPS |
0.50 |
|
Hemin |
0.005 |
|
pH after sterilization ( at 25°C) 7.3 ± 0.2 |
|
Fluid Thioglycollate Medium-
Supplemented w/0.05%SPS
|
Ingredients |
Gms/Liter |
|
Fluid Thioglycollate Medium (DM263) |
29.75 |
|
Yeast Extract |
5.00 |
|
SPS |
0.50 |
MICROMASTER LABORATORIES PVT LTD
ManufacturerIndiaPrimary focus areaMore Products From MICROMASTER LABORATORIES PVT LTD |
